Figure 3.

PQBP1 Nt binding to the hexamer is mediated by charge interactions. (A) and (B) NanoDSF raw melting profiles (left panels) and first derivative curves (right panels) of HIV-1 CA hexamer (0.83 μM) containing non-mutated Arg18 (A) or R18A mutation (B), after incubation with 5 μM Nt peptide (magenta), or Nt DE-to-G mutant (purple), or without peptide (black). Data are representative of at least 3 technical replicates. (C) Summary of thermostabilization data, presented as mean ± S.D. of three independent experiments. (D) Representative 2D class averages of the indicated CA hexamers after incubation with 3-fold excess Nt peptide. Red arrows indicate the central channel.