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. Author manuscript; available in PMC: 2024 Feb 23.
Published in final edited form as: J Mol Biol. 2023 Dec 20;436(4):168409. doi: 10.1016/j.jmb.2023.168409

Figure 5.

Figure 5.

High-affinity hexamer binding requires a tri-aspartate motif in Nt. (A) Sequence of Nt and positions of 15-mer peptides. (B) Binding data for peptides 15–5 and 15–6. Data are representative of three independent experiments, each in three replicates. (C) HIV-dependent ISG54 induction by the indicated PQBP1-eYFP constructs in differentiated THP-1 cells, shown as mean ± S.D. Experiments were done in biological triplicate; shown is representative of two independent experiments. ***, p < 0.001 (two-way ANOVA). (D) Anti-YFP immunoblot of THP-1 whole cell lysates to detect expression levels of indicated PQBP1-eYFP constructs. EV, empty vector.