Figure 6.

PQBP1 binding to the HIV-1 capsid lattice in vitro. (A) Indicated combinations of PQBP1 constructs (50 μM) and disulfide-stabilized CA tubes (50 μM in terms of CA subunits, which is equivalent to 8.3 μM of hexamer) were incubated for 1 h at room temperature, then centrifuged. Load, Supernatant, and Pellet fractions were analyzed using SDS-PAGE and Coomassie staining. Representative of 9 (FL, full-length PQBP1), 3 (ΔNt mutant), 3 (DE-to-G mutant), or 4 (Nt peptide) experiments. (B) Band intensities for pelleted PQBP1 in the presence of CA tubes after subtraction of matching negative controls in the absence of CA tubes, reported as mean ± S.D. (C) FL PQBP1 was incubated with Nt peptide and/or CA tubes as indicated, for 1 h at room temperature prior to centrifugation. Numbers indicate percentage of input FL PQBP1 in the pellet fraction. Representative of 2 independent experiments.