Table 1.
Primer sequences for the bovine FRAS1 mutation loci amplification and qRT-PCR.
| Loci | Rs Numbers | Primer Sequences (5′–3′) | Product Sizes (bp) |
Tm (°C) |
Region | Note |
|---|---|---|---|---|---|---|
| P1-D36-bp | rs438887427 | F1: TTCCACTGTTTCCCCATCTATT | 291/255 | 58.1 | intron 14 | deletion |
| R1: GGCTGTATTTTGTCACCCTTCT | ||||||
| P2-D21-bp | rs432223525 | F2: GCATCCCTGGAATAAACCCAAT | 175/154 | 62.0 | intron 19 | deletion |
| R2: ACCACTACCCTGATACCAAAAC | ||||||
| P3-D20-bp | rs522341234 | F3: GTTAATCGCCCAATATGTCTCGTG | 195/175 | 62.9 | intron29 | deletion |
| R3: CTGAAAGAAGCCTCTCTACCACTC | ||||||
| P4-D15-bp | rs453892138 | F4: ACAGAATTCTCTCCAGAGCAATGAA | 252/237 | 60.3 | exon73 (non-coding region) |
deletion |
| R4: CTGTCTTGGAAGAAACAGTGGC | ||||||
| P5-D18-bp | rs527003260 | F5: GGTCGCAAAGAATTGGACACG | 248/230 | 60.4 | exon 73 | deletion |
| R5: TTGGCAGGTGGGTTCTTAACT | ||||||
| Bovine-FRAS1 | - | F: CACCAGGAGCTGGAATTCAT | 105 | 62.0 | - | qRT-PCR |
| R: AGTCCTCCCATCTTGAAACAC |