Table 1.
Nucleotide sequence of primers used in this study.
| Gene Name | Direction | Primer Sequences (5′–3′) | |
|---|---|---|---|
| Housekeeping gene |
beta-actin | Forward | GCAGGAGTACGATGAGTCCG |
| Reverse | ACGCAGCTCAGTAACAGTCC | ||
| Pluripotent marker genes | NANOG | Forward | TACCTCAGCCTCCAGCAGAT |
| Reverse | CATTGGTTTTTCTGCCACCT | ||
| Oct4 | Forward | CGAACCTGGCTAAGCTTCCA | |
| Reverse | GCCATCCCTCCACAGAACTC | ||
| REX1 | Forward | GCTCCGGCGGAATCGAGTGG | |
| Reverse | GCACGTGTTGCTTGGCGACC | ||
| SOX2 | Forward | CTCGCAGACCTACATGAAC | |
| Reverse | TCGGACTTGACCACAGAG | ||
| Immunomodulatory marker genes | IGF1 | Forward | TGGTGGACGCTCTTCAGTTC |
| Reverse | TCCGGAAGCAACACTCATCC | ||
| TGFB1 | Forward | ATGCCAACTTCTGTCTGGGG | |
| Reverse | GGTTGTAGAGGGCAAGGACC | ||
| IL6 | Forward | CCACCCACAACAGACCAGTA | |
| Reverse | TCTGACAGTGCATCATCGCT | ||
| Adipogenic genes | PPARG | Forward | AGCTCTGTGGACCTCTCTGT |
| Reverse | GTCAGCTCTTGTGAACGGGA | ||
| PDGFRA | Forward | AGTGCTTGGTCGGATCTTGG | |
| Reverse | GAGCATCTTCACAGCCACCT | ||
| FABP4 | Forward | AACTGGGCGTGGAATTCGAT | |
| Reverse | CACATGTACCAGGACCCCAC | ||
| ADIPOQ | Forward | TAATTCAGAGCAGCCCGTAG | |
| Reverse | TGGGGATAACACTCAGAACC | ||
| Osteogenic genes | BSP | Forward | AGGCTACGAGGGTCAGGATT |
| Reverse | GCACCTTCCTGAGTTGAGCT | ||
| OPN | Forward | GAAGAGCCAGGAGTCCGATG | |
| Reverse | CTTCCCGTTGCTGTCCTGAT | ||
| BMP2 | Forward | CAGGTCTTTGCACCAAGATG | |
| Reverse | GCTGGACTTAAGACGCTTCC | ||
| Chondrogenic genes | COL2A1 | Forward | TCCTAAGGGTGCCAATGGTGA |
| Reverse | AGGACCAACTTTGCCTTGAGGAC | ||
| ACAN | Forward | CTCTGCCTCCCGTGAAAC | |
| Reverse | TGAAGTGCCTGCATCTATGT | ||
| SOX9 | Forward | GAAAGACCACCCCGATTACAAG | |
| Reverse | AAGATGGCGTTAGGAGAGATGTG | ||
| Myogenic genes | MyoD | Forward | CGACTCTTCAGGCTTGGGTT |
| Reverse | TGTCGCAAAGGAGCAGAGAG | ||
| MYOG | Forward | GGCAATGCACTGGAGTTTGG | |
| Reverse | CGTAAGGGAGTGCAGGTTGT | ||
| MYF5 | Forward | ATGGACATGACGGACAGCTG | |
| Reverse | TGCGACTCTTGGCTCAAACT | ||