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Mapping the domains of EWS and hTAFII68 which interact with the subunits of TFIID and Pol II. Numbers in the diagrams refer to amino acid positions in either hTAFII68 or EWS. The results of the protein-protein interaction assay, using either baculovirus-overexpressed full-length proteins or E. coli-produced GST fusion proteins, are summarized as follows: +++, ++, +, +/−, and −, strong, moderate, weak, very weak but detectable, and no interactions between the indicated proteins.
