KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Anti-Human IgM | BD Biosciences | Custom, Clone G20–127 |
| Anti-Human CD21 | BD Biosciences | RRID: AB_2738231 |
| Anti-Human CD20 | BD Biosciences | RRID: AB_1727449 |
| Anti-Human IgG | BD Biosciences | RRID: AB_2738683 |
| Anti-Human CD38 | BD Biosciences | Cat# 612969 |
| Anti-Human CD19 | BD Biosciences | RRID: AB_2873553 |
| Anti-Human IgD | BD Biosciences | Custom, Clone IA6–2 |
| Anti-Human CD22 | Biolegend | RRID: AB_2074592 |
| Anti-Human CD27 | Biolegend | RRID: AB_2561450 |
| Anti-Human CD56 | Biolegend | RRID: AB_2561944 |
| Anti-Human CD14 | Biolegend | RRID: AB_2561946 |
| Anti-Human CD3 | Biolegend | RRID: AB_2561943 |
| Anti-Human Hashtag 1 | Biolegend | RRID: AB_2801031 |
| Anti-Human Hashtag 2 | Biolegend | RRID: AB_2801032 |
| Anti-Human Hashtag 3 | Biolegend | RRID: AB_2801033 |
| Anti-Human Hashtag 4 | Biolegend | RRID: AB_2801034 |
| Anti-Human Hashtag 5 | Biolegend | RRID: AB_2801035 |
| Anti-Human Hashtag 6 | Biolegend | RRID: AB_2820042 |
| Anti-Human Hashtag 7 | Biolegend | RRID: AB_2820043 |
| Anti-Human Hashtag 8 | Biolegend | RRID: AB_2820044 |
| Anti-Human Hashtag 9 | Biolegend | RRID: AB_2820045 |
| Anti-Human Hashtag 10 | Biolegend | RRID: AB_2820046 |
|
| ||
| Biological samples | ||
|
| ||
| Human PBMC from US | NIH Blood bank and Duke University | N/A |
| Human PBMC from South Africa | FRESH Cohort | N/A |
| Human PBMC from Rwanda | IAVI | N/A |
| Human PBMC from Uganda | IAVI and MHRP | N/A |
| Human PBMC from Tanzania | MHRP | N/A |
| Human PBMC from Kenya | MHRP | N/A |
| Human PBMC from Thailand | MHRP | N/A |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Streptavidin PE | ThermoFisher | Cat# S21388 |
| Streptavidin APC | ThermoFisher | Cat# S32362 |
| Streptavidin BV711 | BD Biosciences | Cat# 563262 |
| Fixable live/dead stain | ThermoFisher | Cat# L34957 |
| Brilliant Stain Buffer | BD Biosciences | Cat# 563794 |
| Protein A Sepharose | GE LifeSciences | Cat# 17–1279-03 |
| 2-Mercaptoethanol | ThermoFisher | Cat# 21985023 |
| dNTPs | ThermoFisher | Cat# 18427088 |
| TCL Buffer | Qiagen | Cat# 1031576 |
|
| ||
| Critical commercial assays | ||
|
| ||
| Expi293 Expression System Kit | ThermoFisher | Cat# A14635 |
| RNA Clean XP Beads | Beckman Coulter | Cat# A63987 |
| 10x Lysis Buffer | Clontech | Cat# 635013 |
| SMARTScribe RT | Clontech | Cat# 639538 |
| RNAse Inhibitor | Clontech | Cat# 2313B |
| SeqAmp PCR Buffer | Clontech | Cat# 638509 |
| SeqAmp DNA Polymerase | Clontech | Cat# 638509 |
| AMPure XP Beads | Beckman Coulter | Cat# A63881 |
| EXO-CIP Cleanup kit | New England Biolabs | Cat# E1050L |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| Human Expi293F | ThermoFisher | Cat# A14527 |
|
| ||
| Software and algorithms | ||
|
| ||
| Flowjo 10 | FlowJo | https://www.flowjo.com |
| Prism 7/8 | Graphpad | https://www.graphpad.com/ |
| Seaview | RRID: SCR_015059 | |
| Standalone IgBlast | NCBI | https://www.ncbi.nlm.nih.gov/igblast/ |
| Kinetics software | Carterra | https://carterra-bio.com/resources/kinetics-software/ |