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. 2024 Feb 6;13(4):301. doi: 10.3390/cells13040301

Figure 5.

Figure 5

Novel fusion genes involving CRYBG1 in MFM-223. (A) Normalized expression of CRYBG1, FGFR2, and RTN4IP1 visualized as heat map in the 29 BC cell lines from RNA-seq analysis. (B) Alignment of the detected fusion spanning reads of FGFR2::CRYBG1 and RTN4IP1::CRYBG1 to the exons of the fusion partners. (C) Fusion gene validation by PCR with primers flanking the fusion spanning read sequences on cDNA prepared from two biological replicates of RNA and on genomic DNA (gDNA) from MFM-223; amplification of ABL1 served as internal control. (D) Electropherogram after cloning and sequencing of PCR products shown in (C); (a) and (b) indicate the two different FGFR2::CRYBG1 fusions. (E) SNP array results showing focal CNAs (red arrows) on Chr.6q21 and Chr.10q26.13 in MFM-223, genes involved in the fusions (CRYBG1, RTN4IP1, FGFR2) are depicted in red in the enlarged sections. LRR: Log R ratio.