1	Cohort Overview: The cohort of the study is well-defined with regard to how the cohort was recruited and details regarding the diagnosis and treatment of the patients.
2	Clinical Data: Basic clinical data are provided, including the age, gender, clinical stage, and histopathologic grade of the cohort.
3	Test Protocol: For biomarkers not commonly tested in a hospital setting, a detailed protocol for testing the biomarker is provided or referred to.
4	Prognostic Endpoints: The endpoints used for prognostic analysis, such as overall survival or recurrence-free survival, are clearly stated.
5	Statistics: Appropriate statistical analysis is performed to adjust the estimation of the effect of the biomarker for known prognostic factors.
6	Classical Prognostic Factors: The prognostic impact of classical prognostic factors is reported, and the relationship between the evaluated and classical factors is described.