Table 1.

A summary of the main single-cell isolation methods mentioned in this review.

Method	Sample Type	Throughput	Cost	Isolation Efficiency	Automation	Reference
Limited serial dilution	Dissociated cell suspension	Low	Low	Low	No	[13]
FACS	Dissociated cell suspension	High	Very high	Low	Yes	[14]
Micromanipulation	Dissociated cell suspension	Low	Moderate	High	No	[15]
LCM	Tissue	Low	Moderate	High	No	[16]
Microfluidic	Dissociated cell suspension	Very high	High	Moderate	Yes	[17]