FIG. 2.

Identification of gp130 proteins and functions. (A) Parental and transduced H-35 cells were treated for 15 min as indicated. Whole-cell extracts were subjected to EMSA. Binding complexes with the SIE probe and positions of the specific STAT combinations are shown. (B) Lysates from the same cell cultures used in panel A were immunoprecipitated (IP) with anti-FLAG antibodies, and the recovered proteins were separated by SDS-polyacrylamide gel electrophoresis and sequentially reacted in Western blotting (WB) with antibodies against FLAG, phosphotyrosine, and SHP-2 (FLAG, PY, and SHP-2, respectively). H-35 cells contain a protein of 190 kDa (CRM) that cross-reacts with anti-FLAG antibodies.