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. 2024 Feb 7;12(2):383. doi: 10.3390/biomedicines12020383

Figure 4.

Figure 4

Comparison of histological evaluations of retina using H&E staining. (A) Total retinal layer of each group 7 weeks after the initial subconjunctival injections, which was 18 weeks post-diabetes induction (B) Results of total retinal thickness measuring; the DR-MSC-S had a significantly thicker retina compared to DR and DR-MSC groups. (C) Results of INL (yellow arrow) thickness measuring. The DR had the thinnest inner nuclear layer that showed significant difference with INT and DR-MSC-S. There were no statistical differences between INT, DR-MSC, and DR-MSC-S. (D) Results of PRL (white arrow) thickness measuring. The DR had the thinnest PRL that showed significant difference with INT and DR-MSC-S. There were no statistical differences between INT, DR-MSC, and DR-MSC-S. H&E staining, magnification ×200. * p < 0.01. INT, n = 8 rats, 16 eyes; DR, n = 7 rats, 14 eyes; DR-MSC, n = 4 rats, 8 eyes; DR-MSC-S, n = 6 rats, 12 eyes. Abbreviations: H&E, hematoxylin and eosin; NFL, nerve fiber layer; RGC, retinal ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PRL, photoreceptor layer; INT, intact control group; DR, diabetic retinopathy group; DR-MSC, DR group with subconjunctival MSC injection; DR-MSC-S, DR group with subconjunctival sirolimus-pretreated MSC injection.