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. 2024 Feb 6;13(4):304. doi: 10.3390/cells13040304

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Real-time cell proliferation of HNSCC and normal cells under treatment. (A) Exemplary images of HNSCC CAL33 cells under IR ± 5 µM AZD7648 treatment starting from seeding to overgrowth of the surface. (B) Growth curve of normal-shaped CAL33 cells after IR ± KI treatment. An exponential growth equation was fitted from the data points. (C) Doubling time of CAL33 cells in hours under KI ± IR treatment, based on the exponential growth equation above. (D) Doubling time of HSC4, UM-SCC-47 (HNSCC), and HaCaT (healthy keratinocyte) cells in hours.