Figure 7.

Effects of mTOR-Is + IR on the downstream protein S6 in WB. (A) Draft of the effect of IR, Sapanisertib, and CC-115 on the mTOR signaling cascade (created with BioRender.com, accessed on 16 January 2024). (B) Effects of CC115, Sapanisertib, and IR on mTOR signaling cascade in UM-SCC-47 tumor cells. The mean signal intensity of the phosphorylation of S6 (S 235/236) (green) was measured 0, 2, 4, 8, and 16 h after IR with 2 Gy via IS. The experiment was performed with the addition of CC-115, Sapanisertib, or no kinase inhibitor. The cell nuclei in the IS were stained blue using DAPI. Dashed lines indicate the times of the images with p-S6 intensity. Error bars represent the SD of at least three replicates.