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. 2024 Feb 17;46(2):1607–1620. doi: 10.3390/cimb46020104

Figure 2.

Figure 2

Role of MRAP2 on β-arrestin-2 recruitment to PKR1. (A) Concentration–response curves of PKR1-rLuc recorded by BRET assay in CHO cells stably co-expressing GFP-β-arrestin-2 in the absence or presence of MRAP2. Concentration-dependent increase of PKR1-β-arrestin-2 interaction measured in CHO cells stably expressing GFP-β-arrestin-2 after transient transfection with constant amount of PKR1-rLuc in association with either empty or increasing amounts of MRAP2 at a 1:5 or 1:15 receptor/MRAP2 cDNA ratio. (B) Bar graphs of PKR1/rLuc recorded by BRET assay in CHO cells stably expressing GFP-β-arrestin-2 transfected with a constant amount of PKR1-rLuc plus either empty or MRAP2 or 131CT-MRAP2 mutant expressing vectors (1:15 ratio) in the absence of PK2 (−) or after 10 min incubation with PK2 100 nM. Data are the means ± SEM of triplicate values determined in one representative over three independent experiments performed. A Students’ t-test was used for statistical analysis; * p < 0.05, ** p < 0.01 versus basal.