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. 2024 Feb 17;46(2):1607–1620. doi: 10.3390/cimb46020104

Figure 5.

Figure 5

MRAP2 interaction with β-arrestin-2. (A) The GST-fusion protein β-arrestin-2-GST was used to pull down CT-MRAP2 or 131CT-MRAP2 FLAG-tagged. The resulting elution solutions were resolved on 12% SDS-PAGE and analysed by Western blotting with an anti-anti FLAG antibody. I, input; E, eluate. (B) GST-fusion protein hMRAP2-GST was used to pull down β-arrestin-2 or p44. The resulting elution solutions were resolved on 12% SDS-PAGE and analysed by Western blotting with an anti-His antibody. 1. β-arrestin-2 input; 2. p44 input; 3. β-arrestin-2 eluate; 4. p44 eluate. (C) Co-immunoprecipitation experiments using β-arrestin-2 or p44 with His-Tag and CT-MRAP2 with FLAG-tag. Total proteins from E. coli co-expressing CT-MRAP2 FLAG tagged and either β-arrestin-2 (line 1) or p44 (line 2) His-tagged were immunoprecipitated using a FLAG binding resin and the immunoprecipitates resolved by SDS-PAGE. The immunoblots were probed with anti-FLAG (left panel) and anti-His (right panel) antibodies.