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Cellular localization of ZIP kinase. COS-7 cells transiently transfected with the indicated FLAG-tagged ZIP kinase mutant plasmids were indirectly immunostained with anti-FLAG MAb and fluorescein-conjugated secondary antibody and simultaneously stained with DAPI to locate the nucleus. ΔLZ, mouse ZIP-kinase K42A aa 1 to 397; KD, ZIP kinase K42A aa 1 to 275.
