Figure 2.

CBG treatment reduces EGFR and Akt/mTOR protein expression in PDAC cell lines. PDAC cell lines were treated with CBG, and the expression levels of mTOR and EGFR (A), Akt, and pAkt (B) were evaluated by Western blot at 48 h post-treatment. EGFR, Akt, and mTOR densitometric values were normalized to β-actin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which were used as loading controls; pAkt densitometric values were normalized to Akt. Images are representative of one of three separate experiments. Data are expressed as mean ± SD of three separate experiments. * p < 0.05, ** p < 0.01, **** p < 0.0001 treated cells vs. VHC.