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. 2024 Feb 7;25(4):2001. doi: 10.3390/ijms25042001

Figure 4.

Figure 4

CBG reduces the total Pan-RAS expression and the expression of phosphorylated forms of BRAF, CRAF, and MEK1 in PDAC cell lines. (A) PDAC cell lines were treated with CBG, and the expression of Pan-RAS was determined with Western blot at 48 h post-treatment. The densitometric values were normalized to GAPDH used as loading control. Images are representative of one of three separate experiments and data are expressed as the mean ± SD of three separate experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001 treated cells vs. VHC. (B) PDAC cell lines were treated with 12.66 µg/mL CBG for 48 h and the expression of RAS, pBRAF, pCRAF, and pMEK1 was evaluated by Milliplex multiplex assay, and the median fluorescence intensity (MFI) was measured with the Luminex® system. Data shown are expressed as the mean ± SD of three separate experiments. ** p < 0.01, *** p < 0.001, **** p < 0.0001 treated cells vs. VHC.