Figure 6.

CBG increased the cytotoxic effect of chemotherapeutic drugs in PDAC cell lines. (A,B) Cell viability was determined in PDAC cell lines by MTT assay. Cells were treated for 72 h with CBG alone or in combination with different doses of GEM and PTX. Data shown are expressed as mean ± SD of three separate experiments. **** p < 0.0001 CBG combined with chemotherapeutic drug vs. chemotherapeutic drug alone. (C,D) Drug interaction of CBG with PTX and GEM in PDAC cell lines. Effect of single and combined treatments with CBG (7.91, 12.66, 15.82 µg/mL), PTX (1.5, 3, 6 µg/mL), and GEM (25, 50, 100 µg/mL) on cell viability of PDAC cell lines. Drug interaction was evaluated with SynergyFinder software using the Bliss model. Bliss synergy score larger than 10 is considered synergistic, a score from −10 to 10 is considered additive, and a score less than −10 is considered antagonistic.