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Radical point mutations in TBP affect binding of BRF and B". (A) Gel mobility shift assay of each TBP mutant, either alone (T) or with wild-type BRF (B), C-BRF352 (C2), or C-BRF309 (C9). (B) TFIIIB complex formation of TBP mutant E93R and wild-type (WT) TBP. TBP was added alone (T), with BRF (B), or with BRF and 24 ng of B" (B").
