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Primer sequences and annealing temperatures used for PCR and RT-qPCR.
| Gene | Primer Sequences (5′–3′) | Size (bp) | Annealing Temperature (°C) |
Use |
|---|---|---|---|---|
| KRT84 | F: CCATCATGTCTTGCCGCTCCT | 587 | 60 | PCR |
| R: ACACCCCAGTGCATTTAGCTC | ||||
| KRT84 | F: ACGATCTTCAGTGGCCCAAG | 104 | 60 | RT-qPCR |
| R: GGGGTTAAACCAACACCCCA | ||||
| ACTB | F: AGCCTTCCTTCCTGGGCATGGA | 113 | 60 | RT-qPCR |
| R: GGACAGCACCGTGTTGGCGTAGA |
