FIG. 8.

The CD5 receptor is associated with p85 (PI 3-kinase) and Vav. T cells were left unstimulated or stimulated with PHA plus anti-CD28 (αCD28) in the presence or absence of anti-CD5 (αCD5) for 5 min. CD5 was immunoprecipitated from total-cell lysates, and CD5, p85, and Vav were detected by ECL Western blotting as described in Materials and Methods.