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[Preprint]. 2024 Feb 14:2024.02.14.580389. [Version 1] doi: 10.1101/2024.02.14.580389

Figure 4: ALKBH1 regulates mitochondrial function through its dioxygenase activity.

Figure 4:

a: Schematic for the synthesis pathway of hm5C and f5C modifications and the responsible enzymes. b: Validation of ALKBH1 knockout (KO) in the two generated clones. c: Analysis of ALKBH1 expression after 8 hours of stress exposure. d: LCMS/MS analysis of tRNA modifications after ALKBH1 KO. Data is normalized to Mock. Asterisk indicates fold change > 1.5 and p < 0.05. e: Analysis of hm5C peaks showing the complete depletion of hm5C after ALKBH1 KO. f: Cell proliferation after ALKBH1 KO. g: Analysis of OXPHOS related proteins in all 5 complexes using western blot. h: Mitochondrial respiration analysis using Seahorse.