Fig. 5: 43 stabilizes ChREBPα/14-3-3 interaction and thus retains cytoplasmic ChREBPα localization in response to glucose and glucolipotoxicity.

a. Proximity ligation assay demonstrating increased interaction between 14-3-3 and ChREBPα. INS-1 cells were cultured overnight (ON) at low (5.5 mM) glucose and exposed to high (20 mM) glucose for the indicated times b, d. Representative figures showing the nuclear localization of ChREBPα after exposure to high glucose (b) or glucolipotoxic (d) conditions. c, e. Time course of nuclear localization of ChREBPα based on figures b, d, respectively in addition to 1mM AICAR. f, g. CRISPR/Cas9 engineered INS-1 cells treated with the indicated compounds for 24 h; Low-5.5 mM glucose, High-20 mM glucose, glucolipotoxicity-20 mM glucose+500 μM palmitate. f. Representative images at 24 h. g. Quantification of % nuclear ChREBP at 24 h. Data are the means +/− SEM, n=3–5, *p<0.05, **p<0.01.