TABLE 2.
Effect of PSS end filling on REMIa
| Digesting enzyme | End filling | Added enzyme | Relative frequency of integration ± SE | Fold increase |
|---|---|---|---|---|
| Asp718 | − | KpnI | 3.10 ± 0.28 (68) | 1.9b |
| Asp718 | + | 2.36 ± 0.52 (52) | ||
| Asp718 | + | KpnI | 3.26 ± 0.56 (78) | 1.4b |
| BglII | − | 1.63 ± 0.30 (36) | ||
| BglII | − | BamHI | 6.34 ± 1.79 (140) | 3.9b |
| BglII | + | 3.10 ± 0.28 (64) | ||
| BglII | + | BamHI | 2.18 ± 0.52 (48) | 0.7 |
| SalI | − | 1.63 ± 0.25 (37) | ||
| SalI | − | BamHI | 8.88 ± 1.28 (196) | 5.5b |
| SalI | + | 3.08 ± 0.26 (44) | ||
| SalI | + | BamHI | 3.01 ± 0.27 (63) | 1 |
a
The plasmid pM151 was digested with restriction enzymes, and the ends were filled in with Klenow and deoxyribonucleoside triphosphates. The frequency of REMI has been determined in strain RSY12 as described in footnote b to Table 1. The data are representative of four independent experiments.
b
t test indicates a statistically significant difference (P < 0.05) from control values.