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. 2024 Feb 9;25(4):2123. doi: 10.3390/ijms25042123

Figure 2.

Figure 2

miR-34a expression in PCa cells and xenografts and miR-34a mimic downregulated molecular targets and inhibited PCa cell growth. (A) miR-34a-5p expression levels in four pairs of PCa xenografts and indicated PCa cell lines. AD, androgen-dependent; AI, androgen-independent. (B) miR-34a-5p expression 48 h post-transfection of PC3 cells with 30 nM miR-34a mimic or NC (n = 3). (C) Targeted silencing of miR-34a Renilla sensor using miR-34a mimic in PC3-miR-34a sensor cells in vitro. Data points were normalized to NC (n = 3). (D) Evaluation of the expression of miR-34a targets by qRT-PCR from PC3 cells following transfection with 30 nM miR-34a mimic for 48 h. (E) Representative Western blot images depicting downregulation of c-Myc, Cyclin D1, or CD44 expression following transfection of PC3 cells with miR-34a (n  =  3). (F) Quantitative data from E normalized to respective NC. (G) Effect of miR-34a mimic on PC3 cell growth measured by trypan blue cell counting 120 h after transfection of the indicated conditions. (n = 3). In all experiments, miR-34a mimic or NC were transfected into PC3 cells using lipofectamine RNAiMax. *, p < 0.05; **, p < 0.01; ****, p < 0.0001 compared to respective NC.