Table 3.

Manual validation of orthogroups not present in all species examined in Figure 5.

Venn Group	CmBEI	CmUKMEL1	CpBGF	Gene Product	Findings
3			cpbgf_7005600	GMP synthase	Subtelomeric – cannot conclude if in syntenic regions of CmBEI or CmUKMEL1
			cpbgf_1003900	GMP synthase	Subtelomeric in C. parvum, as above
			cpbgf_1003890	GMP synthase	Subtelomeric in C. parvum, as above
	Found cmbei_2003270	Found cmeUKMEL1_06080	cpbgf_2003270	phosphoglucomutase	Orthofinder created two separate groups for this single gene (see below)
			cpbgf_1003950	tryptophan synthase beta chain	Subtelomeric in C. parvum – cannot conclude if in syntenic regions of CmBEI or CmUKMEL1
		cmeUKMEL1_09025	cpbgf_200460	unspecified product	Subtelomeric in C. parvum, as above
1	Found cmbei_7005006	cmeUKMEL1_03235	Not found	hypothetical protein	Exists in CmBEI – annotation added
11
	cmbei_2003270	cmeUKMEL1_06080	Found: cpbgf_2003270	phosphoglucomutase/phosphomannomutase alpha/beta/alpha domain I family protein	Orthofinder created two separate groups for this single gene (see above)
	cmbei_2003445	cmeUKMEL1_05990	Found	hypothetical protein, only 78 aa	No start methionine in C. parvum. Limited expression evidence in C. parvum
	cmbei_3002025	cmeUKMEL1_12210	Found cpbgf_3002017	Rpp14/Pop5 family protein	Exists as noncoding RNA in C. parvum IOWA BGF
	cmbei_4002857	cmeUKMEL1_09770	Found	hypothetical protein	Exists in C. parvum IOWA BGF, Unannotated
	cmbei_4003225	cmeUKMEL1_09950	Not found	hypothetical protein, only 71 aa	Gene cannot be found in C. parvum IOWA BGF syntenic region
	cmbei_400495	cmeUKMEL1_08280	Not found	hypothetical protein, only 78 aa	Exists in C. parvum IOWA BGF, Unannotated
	cmbei_500300	cmeUKMEL1_09795	Found	hypothetical protein	Exists in C. parvum IOWA BGF, Unannotated
	cmbei_110040	cmeUKMEL1_17820	Found	hypothetical protein	Exists in C. parvum IOWA BGF, Unannotated
	cmbei_6004835	cmeUKMEL1_05455	Found	hypothetical protein	Exists in C. parvum IOWA BGF, Unannotated
	cmbei_7001355	cmeUKMEL1_10685	Found	hypothetical protein, only 64 aa	There is a stop codon in the middle of the 3’ ORF
	cmbei_100030	cmeUKMEL1_09030	Found cpbgf_200445	putative integral membrane protein	Exists as long noncoding RNA in C. parvum IOWA BGF

aa = amino acids