FIG. 3.

In vitro complex formation between mutant BKV TAgs and proteins pRb and p107. Whole cell-lysate (150 μg) from BSC-tet cells transiently transfected with pBKT-tet, pE109K-tet, and pH42Q-tet were incubated with equivalent amounts of each purified GST-Sepharose-bound fusion protein. Bound complexes were released and separated by SDS-PAGE, transferred to nitrocellulose, and probed with PAb416. For whole-cell lysate samples (WCL), 50 μg of lysate from each cell line was used.