Figure 3. MFS is localized in the mitochondria and cell surface of S. brasiliensis yeast.

S. brasiliensis yeast cells were exposed to fluorescent MFS (2μg/mL) for 1 h and then stained with MitoTracker Deep Red FM. The MFS and MitoTracker signals merge on the mitochondria, while the MFS signal is observed on the cell surface. Three independent experiments were performed, and 100 cells were counted for each to calculate a 47.06 ± 1.01 % of MFS and MitoTracker colocalization (merge).