Figure 2:

Cell-type chromatin-accessibility bias for core enzymes in C₄ and C₃ species. A) Schematic of the core C₄ enzymatic pathway. Core C4 enzymes are defined as those which maintain their cell-type-specificity in all C₄ subtypes sampled. The red and blue squares represent MS and BS cells, respectively. Enzymes are labeled in bold, and transporters are denoted by shapes. Intermediate molecules are indicated by non-bolded text. B) Screenshot of PEPCK in Z. mays. Blue tracks correspond to BS chromatin accessibility and red tracks show MS chromatin accessibility. Tracks are equally scaled to facilitate comparison. C) Heatmaps of chromatin accessibility bias of the core C₄ enzymes. Values within each heatmap correspond to Log2(BS/MS). Blue indicates increased BS chromatin accessibility and red indicates increased MS chromatin accessibility. Each species column and subtype was clustered independently, and genes were assigned as being MS- or BS-specific (top/bottom of heatmap) based on literature. Enzyme copies were distinguished phylogenetically.