Fig.5 |. GRIP1 is required for the establishment of M2_IL4 and M2_GC transcriptomes.

a-b, Expression of (a) GRIP1 or (b) IL4- (top) GC- (middle) or shared (bottom) M2 target genes in WT and GRIP1 cKO M0, M2_IL4, M2_Cort, and M2_Dex populations as assessed by RT-qPCR. Average relative expression of each transcript in WT M0 is arbitrarily set to 1. n=4; Student’s t-test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, non-significant. Error bars are SEM. c, Stacked bar plot shows the number of DEGs in GRIP1 cKO vs. WT as a fraction of WT M2_IL4, M2_Dex, or shared DEGs relative to M0 (RNAseq, WT: n=3, cKO: n=4). d, Volcano plot shows DEGs in GRIP1 cKO vs. WT in M2_IL4 (left) and M2_Dex (right) relative to M0 of each genotype. (FC≥1.5, unadjusted p-value<0.05). Selected inflammation-related genes expressed at higher levels in the cKO M2_IL4 and M2_Dex relative to WT are highlighted in red. Key M2 target genes downregulated in the cKO are highlighted in both M2_IL4 (orange) and M2_Dex (green). Examples of the shared M2_IL4:M2_Dex GRIP1 target genes are underlined. e, Differentially regulated pathways (unadjusted p < 0.01) identified using QuSAGE with MsigDB canonical pathways subset (Broad Institute) are shown for GRIP1 cKO vs. WT in M2_IL4 (left) and M2_Dex (right). Select pathways are labeled (upregulated, red; downregulated, orange for IL4 and green for Dex); M2_IL4:M2_Dex-shared pathways are underlined. Circle size is proportional to the number of genes in the pathway and color signifies p-value.