Fig.6 |. Macrophage GRIP1 contributes to phagocytic activity of M2_IL4 and M2_GC in vitro and tissue healing in vivo.

a, Relative fluorescence intensity of WT (set to 100%) and GRIP1-cKO M2_IL4, M2_Cort and M2_Dex relative to that in M0 after exposure to pHrodo Green S. aureus BioParticles (see Methods). Shown are mean values and error bars are SEM (n=3, student t-test). b, Histopathological changes assessed by H&E staining of colons from WT and cKO mice 2 wks after initiating DSS-induced colitis. Grade 1, 2 and 3 are defined in Methods. Plotted is the total area representing grade 3 for colon specimens of WT and GRIP1-cKO (n=4, student t-test). c, The expression of indicated genes was measured by RT-qPCR of total RNA from colons of WT and cKO mice. The expression of each gene was normalized to β-actin, and then fold difference to baseline, non-treated (calibrant) value calculated for WT or cKO. Shown are mean values and error bars are SEM (WT: n=10–15, cKO: n=6–8; student t-test).