Figure 7. The tRNA splicing factors TSEN2 and CLP1 modulate 5’ pre-tDR-Tyr expression during MHV68 infection.

siRNA-mediated knockdown of Tsen2 (siTsen2) and Clp1 (siClp1) or treatment with non-targeting siRNAs (siNT) was followed by mock or MHV68 infection at an MOI=5 for 24 h. (A) siRNA knockdown was confirmed using RT-qPCR using Clp1, Tsen2, and 18S-specific primers. (B) SL-qPCR was used to measure 5’ pre-tDR-Tyr upon siRNA treatment. Data from RT-qPCR and SL-qPCR experiments is depicted as mean +/− SD from four independent experiments relative to the siNT/mock sample, with p-values calculated using raw DCt values and paired t-test. (C) Viral titer of supernatants was measured by TCID50 from three independent experiments, with p-value calculated by one-way ANOVA. ns = p>0.05; * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001