FIG. 3.

Formation of His₆-Rbl2p–β-tubulin complex in vitro. Bacterial extract containing His₆-Rbl2p was incubated with wild-type yeast cell extracts at 4°C. At various times, an aliquot was removed and added to Ni-NTA beads for 15 min. The beads were washed, and the bound proteins were assayed by elution followed by immunoblotting with anti-tubulin antibodies. The data are reported as percent β-tubulin and α-tubulin bound as a function of time.