Figure 3.

Cisplatin increases gene expression of pro-inflammatory cytokines. The relative gene expression of pro-inflammatory cytokines (il12, il8, and il6), anti-inflammatory cytokine (il10), and renal damage marker (kim1) was measured in larvae at 9 dpf. The larvae were exposed to 0.10 mg/mL of cisplatin (Cisp) or embryonic medium (Ctr) for 48 h. The data show that cisplatin results in increased expression of inflammatory markers. Means were obtained from 6 to 9 samples, which referred to a pool of 20 larvae per sample. A t-Student test was performed between the cisplatin groups and the control group for each gene, with significance indicated as *** p = 0.0030; # p = 0.0001; and & p = 0.0382; ns = not significant, p > 0.05. The data are presented as mean and standard deviation.