Figure 6.

Cisplatin exposure induces cell death in pronephros. Larvae AB (wild type) at 9 dpf were exposed to 0.10 mg/mL of cisplatin (Cisp) or embryonic medium (Ctr) for 48 h. The number of positive cells in the glomerular region of the pronephros indicating cell death was quantified using the TUNEL assay. (A). Representative images showing cell nucleus marking with Hoechst (in blue) and cell death marking (in red). Rostral on the left, dorsal above. (B). The number of TUNEL-positive cells in the glomerular region of the pronephros (circled in white in (A)) was increased. The t-Student test was performed between the groups (* p = 0.0483). Data are presented as mean and standard deviation.