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Telomerase expression in immortalized MSCs. (a) TERT mRNA expression level before (pMSCs) and after the immortalization procedure (iMSC-1 and iMSC-2) established using qPCR; (b) Western blot analysis of TERT protein content; (c) telomerase activity assessment by qPCR assay; (d) relative telomere length. *—p < 0.05 (ANOVA multiple pairwise comparison, Tukey test), n = 3. Data are presented as mean ± standard deviation.
