Figure 5.

iMSC-2 differentiation potential. (a) MSCs cultured in appropriate differentiation medium for 2 weeks. Adipogenic differentiation was assessed by Nile red staining of accumulated lipids (green fluorescence), osteogenic differentiation—by Alizarin red staining of calcium deposits (red color) and chondrogenic differentiation—by Alcian blue staining of cartilage acidic glycosaminoglycans (light blue color); (b) relative expression level of marker genes of osteogenic (osteocalcin, Runx) and adipogenic (PPARgamma, adiponectin) differentiation in pMSCs and iMSCs cultured for 15 days in ordinary complete growth medium or appropriate differentiation medium.