Figure 7.

α-Tubulin peptide increases grA lifetime: (A) Current traces of grA channels in DOPE membrane before (Control) and after the addition of 10 and 20 μM of α-tubulin peptide to both sides of the DOPE membrane. Current records were filtered with a digital 8-pole Bessel filter at 1 kHz. Dashed lines indicate zero current level. The membrane bathing solution contained 1 M KCl buffered with 5 mM HEPES at pH 7.4. The applied voltage was 100 mV. α-tubulin peptide was dissolved in DMSO. (B) α-tubulin peptide increases grA lifetime in a dose-dependent manner. Bars and error bars are the mean and standard deviation from the mean; the symbols represent data points of 4 independent experiments. Control measurements with the addition of DMSO aliquots corresponding to 20 μM of α-tubulin peptide addition do not show the effect on grA lifetime.