Figure 2.

Effect of ApoE isoforms on S100A9 amyloid morphology observed by AFM imaging. AFM images of S100A9 amyloids formed (A,F) without ApoE isoforms and in the presence of (B,G) 5 µM ApoE3; (C,H) 5 µM ApoE4; (D,I) 4 µM ApoE3-DOPC; and (E,J) 4 µM ApoE4-DOPC after 15 h and 70 h incubation, respectively. A total of 50 µM S100A9 was aggregated in PBS, containing 1 mM sodium azide and in non-stirring condition at 42 °C. Scan sizes are 2 × 2 μm. The z-scale in AFM images is indicated by a bar with a color gradient from black to light yellow shown on the right of the images.