Table 6.
mRNA primer sequence for OA-induced cartilage tissues.
| MMP-1 | F | AACTTGGGTGAAGACGTCCA |
| R | TCCTGTCACTTTCAGCCCAA | |
| MMP-3 | F | GTACGGCTGTGTGCTCATCC |
| R | TCAGCCCAAGGAACTTCTGC | |
| MMP-8 | F | TCTGTTCTTCTTCCACACACAG |
| R | GCAATCATAGTGGCATTCCT | |
| MMP-13 | F | ACCTTCTTCTTGTTGAGTTGGA |
| R | CTGCATTTCTCGGAGTCTA | |
| JUN | F | CCAACCAACGTGAGTGCAAG |
| R | GAG GGCATCGTCGTAGAAGG | |
| FOS | F | TACTACCATTCCCCAGCCGA |
| R | GCGTATCTGTCAGCTCCCTC | |
| IL-1β | F | AACTCAACTGTGAAATAGCAGC |
| R | TCCACAGCCACAATGAGTG | |
| TNF-α | F | GCATGATCCGAGATGTGGAA |
| R | GATGAGAGGGAGCCCATTTG | |
| IL-6 | F | TCCGCAAGAGACTTCCAGC |
| R | CCTCCGACTTGTGAAGTGG | |
| COX-2 | F | GTTCCAACCCATGTCAAAAC |
| R | TGTCAGGAATCTCGGCGTAG | |
| Ptger2 | F | TGTGTGTACTGTCCGTCTGC |
| R | CAGGGATCCAGTCTCGGTGT | |
| TGF-β1 | F | AGGAGACGGAATACAGGGCT |
| R | CCACGTAGTAGACGATGGGC | |
| Type II collagen | F | TGGCCTTGGTGGAGGAAA |
| R | AGGACCAGGGAGGCCTCTTT | |
| IL-4 | F | CGTGATGTACCTCCGTGCTT |
| R | GTGAGTTCAGACCGCTGACA | |
| TIMP-1 | F | TTTCCCTGTTCAGCCATCCC |
| R | TAGCCCTTCTCAGAGCCCAT | |
| GAPDH | F | CTTGTGACAAAGTGGACATTGTT |
| R | TGACCAGCTTCCCATTCTC |
The mRNA primer sequence used the Rattus norvegicus gene. MMP: matrix metalloproteinase, JUN/FOS: gene of activator protein-1, IL: interleukin, TNF: tumor necrosis factor, COX: cyclooxygenase, Ptger2: prostaglandin E receptor 2, TGF: transforming growth factor, TIMP: tissue inhibitor of metalloproteinase, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.