Table 1.
Predicted G-protein-coupled receptor-like proteins in M. grisea
| Known receptors used as query in BLAST against M. grisea proteins or another search method | M. grisea proteins retrieved by known receptor (BLASTP) | E-value | Other proteins homologous to M. grisea proteins retrieved by known receptor | PfamA GPCR domains (E-value)/conserved domain identified in the present study |
| Pheromone receptor (CAC86431; STE2-like) | MG04711.4* | 3e-65 | Pfam STE2 (2.1e-04) | |
| Pheromone receptor STE3 (STE3_YEAST) | MG06452.4† | 2e-14 | Pfam STE3 (1.1e-09) | |
| cAMP receptor TASA (Q9NDL2) | MG06738.4* ,† | 5e-11 | Pfam7tm_2 (1.3e-04)/cAMP_dom | |
| MG06797.4 | cAMP_dom | |||
| MG06257.4* | cAMP_dom | |||
| MG00326.4 | Pfam 7tm_2 (7.9e-05)/cAMP_dom | |||
| MG00258.4 | cAMP_dom | |||
| MG10544.4 | cAMP_dom | |||
| GPCR GPR1 (GPR1_YEAST) | MG08803.4 | 4e-18 | ||
| GPCR STM1 (STM1_SCHPO) | MG04698.4* | 5e-19 | STM1_dom | |
| MG02855.4* ,† | 1e-17 | STM1_dom | ||
| GPCR mPR (NP_848509) | MG05072.4* | 6e-17 | mPR_dom | |
| MG09091.4* | mPR_dom | |||
| MG04679.4* ,† | mPR_dom | |||
| PTH11 receptor (AF119670_1) | MG05871.4 (PTH11) * ,†,‡ | 0 | PTH11_dom | |
| MG10473.4‡ | 3e-34 | PTH11_dom | ||
| MG06755.4‡ | 1e-33 | PTH11_dom | ||
| MG07553.4‡ | 2e-32 | PTH11_dom | ||
| MG09022.4* ,‡ | 2e-27 | PTH11_dom | ||
| MG07565. * ,†,‡4 | 6e-23 | PTH11_dom | ||
| MG07946.4†,‡ | 3e-21 | PTH11_dom | ||
| MG11006.4 | 2e-32 | PTH11_dom | ||
| MG09070.4* | 2e-29 | PTH11_dom | ||
| MG07806.4 | 2e-21 | PTH11_dom | ||
| MG03584.4† | 1e-22 | PTH11_dom | ||
| MG05214.4* | 4e-31 | PTH11_dom | ||
| MG09863.4* ,‡ | 1e-28 | PTH11_dom | ||
| MG10407.4* | 3e-26 | PTH11_dom | ||
| MG10571.4* ,† | 4e-25 | PTH11_dom | ||
| MG01867.4‡ | 1e-23 | PTH11_dom | ||
| MG09455.4†,‡ | 2e-23 | PTH11_dom | ||
| MG10050.4‡ | 1e-14 | PTH11_dom | ||
| MG09667.4 | 1E-22 | PTH11_dom | ||
| MG05352.4* | 2e-22 | PTH11_dom | ||
| MG07420.4 | 1e-21 | PTH11_dom | ||
| MG10442.4 | 4e-20 | PTH11_dom | ||
| MG02160.4† | 6e-19 | PTH11_dom | ||
| MG02001.4* ,† | 1e-18 | PTH11_dom | ||
| MG10257.4 | 2e-18 | PTH11_dom | ||
| MG01905.4 | 2e-17 | PTH11_dom | ||
| MG07987.4 | 1e-16 | PTH11_dom | ||
| MG10438.4* ,‡ | 6e-18 | PTH11_dom | ||
| MG06171.4* | 1e-17 | PTH11_dom | ||
| MG07851.4 | 1e-17 | PTH11_dom | ||
| MG04935.4* | 1e-17 | PTH11_dom | ||
| MG05386.4 | 3e-17 | PTH11_dom | ||
| MG09865.4* ,† | 3e-16 | PTH11_dom | ||
| MG09061.4 | 4e-16 | PTH11_dom | ||
| MG05514.4* ,† | 1e-16 | PTH11_dom | ||
| MG06535.4* | 3e-14 | PTH11_dom | ||
| MG01190.4 | 7e-14 | PTH11_dom | ||
| MG10581.4* | 7e-14 | PTH11_dom | ||
| MG03009.4* | 2e-13 | PTH11_dom | ||
| MG10747.4 | 8e-13 | PTH11_dom | ||
| MG03935.4 | 2e-12 | PTH11_dom | ||
| MG04682.4* | PTH11_dom | |||
| MG09416.4 | 1e-10 | PTH11_dom | ||
| MG02692.4* | 2e-10 | PTH11_dom | ||
| MG07857.4 | PTH11_dom | |||
| MG00826.4 | PTH11_dom | |||
| MG06624.4* ,† | PTH11_dom | |||
| MG00435.4* | PTH11_dom | |||
| MG08653.4* | PTH11_dom | |||
| MG10706.4* ,† | PTH11_dom | |||
| MG04170.4* | PTH11_dom | |||
| MG08525.4* | PTH11_dom | |||
| MG00277.4*,† | PTH11_dom | |||
| MG02365.4* | PTH11_dom | |||
| MG06595.4 | PTH11_dom | |||
| MG06084.4* | PTH11_dom | |||
| MG09437.4* | PTH11_dom | |||
| MG01890.4 | PTH11_dom | |||
| MG01871.4 | PTH11_dom | |||
| MG03794.4 | PTH11_dom | |||
| MG01884.4* | PTH11_dom | |||
| InterProScan MG00532.1 | MG00532.4 (weak similarity to animal GPCRs) * | Pfam 7tm_2 (1.4e-02) |
Classes of GPCR-like protein in M. grisea were subdivided on the basis of BLASTP analysis and shared domains, as described in Materials and methods. They were clustered into paralogous families if the proteins showed 30% identity and 80% overlap over the complete length of the protein. Paralogous families are separated by a blank line. The GPCR-like proteins in M. grisea could be classified into nine subclasses containing more than one member and 48 containing a single member. Six subclasses contained two members, two contained three and one contained six. *M. grisea proteins represented by genes expressed in microarray experiments. †M. grisea proteins that are represented in M. grisea ESTs. ‡Proteins containing the cysteine-rich CFEM domain.