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ABF-1 mRNA is highly abundant in activated human B cells. Northern blot analysis of 10 μg of total RNA isolated from purified peripheral blood B lymphocytes activated in vitro. B cells were treated with SAC for 3 days or with SAC and IL-2 for 6 days or were left untreated. The Northern blot was probed with the ABF-1 cDNA (top), stripped, and then reprobed with elongation factor 1 alpha (EF-1α; bottom) as a control.
