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ABF-1 binds to an E box in vitro. (A) EMSA analysis of FLAG ABF-1 homo- and heterodimeric complexes formed on the μE4-OCT probe. Arrows indicate the position of ABF-1 homodimers and ABF-1–E2A heterodimers. retic., reticulocyte lysate; FP, free probe; NS, nonspecific complex; S, supershifted complex. (B) ABF-1 binds as a heterodimer to the μE4 probe. Addition of a nonspecific control antibody had no effect on complex formation (not shown).
