Table 3. Primer sequences for qRT-qPCR.
Gene | Forward 5’-3’ | Reverse 5’-3’ |
---|---|---|
miR-328-3p | AACAATCTGGCCCTCTCTGC | CAGTGCAGGGTCCGAGGT |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
TLR2 | AAGCAGCATATTTTACTGCTGG | CCTGAAACAAACTTTCATCGGT |
TLR3 | CCTGGTTTGTTAATTGGATTAACGA | TGAGGTGGAGTGTTGCAAAGG |
TLR4 | TTTGGACAGTTTCCCACATTGA | AAGCATTCCCACCTTTGTTGG |
TLR9 | TGGTGTTGAAGGACAGTTCTCTC | CACTCGGAGGTTTCCCAGC |
P65 NF-κB | AACAGAGAGGATTTCGTTTCCG | TTTGACCTGAGGGTAAGACTTCT |
NLRP3 | CGTGAGTCCCATTAAGATGGAGT | CCCGACAGTGGATATAGAACAGA |
IL-1β | ATGATGGCTTATTACAGTGGCAA | GTCGGAGATTCGTAGCTGGA |
IL-18 | GCTGAAGATGATGAAAACCTGG | CAAATAGAGGCCGATTTCCTTG |
β-actin | CCTGGCACCCAGCACAAT | GGGCCGGACTCGTCATAC |