TABLE 5.
Conservation of putative Cys boxes of Rpn11 homologs
| Group | Species | Locus or named | Cys boxa | Total amino acids | % Identity to Rpn11b |
|---|---|---|---|---|---|
| Ic | Saccharomyces cerevisiae | YFR004w (Rpn11) | 104 VVGWYHSHPGFGCWLSSVDVNTQ 126 | 306 | 100 |
| Caenorhabditis elegans | U00032 | 106 ----------------------- 128 | 319 | 52 | |
| Discoides discoideum | U96916 | 108 -I--------------------- 130 | 306 | 58 | |
| Schizosaccharomyces pombe | D31731 (pad1) | 107 -----N-------------I--- 129 | 308 | 64 | |
| Homo sapiens | U86782 (Poh1) | 108 ----------------G--I--- 130 | 310 | 65 | |
| Mus musculus | Y13071 | 107 ----------------G--I--- 129 | 309 | 65 | |
| S. mansoni | AF014465 | 111 ----------------G--M--- 133 | 313 | 65 | |
| IIc | C. elegans | U80814 | 134 ----------Y-----GI--S-- 156 | 368 | 27 |
| Arabidopsis thaliana | AF000657 | 137 ----------Y-----GI--S-- 159 | 357 | 29 | |
| H. sapiens | U70734 (Jab1) | 133 AI--------Y-----GI--S-- 155 | 334 | 29 | |
| M. musculus | U70736 | 133 AI--------Y-----GI--S-- 155 | 334 | 29 | |
| S. cerevisiae | YDL216c (Z74264) | 174 ----F-----YD----NI-IQ-- 196 | 455 | 23 |
a
The putative active-site cysteine is marked in boldface. Dashes represent identities to Rpn11.
b
The identity of the product of each hypothetical ORF to Rpn11 was determined by the Jotun Hein method (gap penalty, 11; gap length, 3) with MegAlign. The identity within the putative Cys box was greater than that over the entire ORF.
c
A pairwise comparison of all proteins indicated that the proteasomal subunits in group I were 52 to 65% identical to each other. Similarly, putative members of group II from different phyla were up to 60% identical to each other. Identity between proteins from different groups was slightly less than 30%.