Figure 1.

Block of TMEM16A chloride currents by Ani9. (A) Representative currents elicited by 1000 ms test potentials between −100 and +80 mV in the same myocyte before (Ctrl, black) and after the addition of 1 µM Ani9 (+Ani9, gray) and the corresponding Ani9-sensitive currents (magenta) calculated by subtraction. On top, the stimulation protocol used to elicit currents. (B) The I-V relationships in absence and presence of Ani9. (C) The histogram shows the blockage exerted by Ani9 that was significant only for the outward currents at +80 mV (n = 8, ** p = 0.002).