Figure 4.

The effect of Ani9, a TMEM16A antagonist, on the myocyte fusion index. (A) Representative optical fields where nuclei are stained in 3-day myocyte cultures in control conditions and in the presence of 5 μM Ani9. Nuclei staining is shown as DAPI fluorescence overlay on corresponding bright-field images. Myotubes are indicated by white arrowheads. Scale bar, 50 μm. (B) A reduction in the mean fusion index was observed at the concentrations of 5 μM (**** p < 0.0001) and 10 µM Ani9 (**** p < 0.0001) with respect to control. Ani9 3 μM vs. 5 μM, p < 0.0001; Ani9 3 μM vs. 10 μM, p < 0.0001; Ani9 5 μM vs. 10 μM, p = 0.0005.