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. 2024 Feb 16;25(4):2344. doi: 10.3390/ijms25042344

Figure 4.

Figure 4

Phenylephrine-induced cardiomyocyte hypertrophy was inhibited by Marine Active® in cultured cardiomyocytes. (A,B) Effects of Marine Active® on primary cultured cardiomyocytes. (A) Representative images of immunofluorescence staining for anti-α-actinin (red) and Hoechst 33258 (blue) in primary cultured cardiomyocytes. Scale bar: 20 μm. (B) The results of cardiomyocyte surface area. All data are presented as mean ± SD (n = 4). (C,D) The results of in vitro p300-HAT assay using 10 mM anserine, 10.6 mM L-histidine, 1.0 mM carnosine and 6.3 mg/mL Marine Active® (10 mM anserine, 10.6 mM L-histidine and 1.0 mM carnosine). (C) Representative photos of Western blotting in vitro. Western blotting was performed with anti-acetyl-histone H3K9 and anti-histone H3 antibodies. (D) Concentration–response curve of acetyl-histone H3K9/histone H3 vs. common logarithm (concentrations). The IC50 value for the inhibition of p300 HAT activity with Marine Active® was 4.64 mg/mL (2.70 mM anserine) (n = 3).